tap antibody Search Results


anti sca1 pe  (Miltenyi Biotec)
94
Miltenyi Biotec anti sca1 pe
Anti Sca1 Pe, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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antibody against sca 1  (Miltenyi Biotec)
94
Miltenyi Biotec antibody against sca 1
Antibody Against Sca 1, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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tap antibody  (Santa Cruz Biotechnology)
92
Santa Cruz Biotechnology tap antibody
Tap Antibody, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti tap nxf1 antibody  (Proteintech)
93
Proteintech anti tap nxf1 antibody
Anti Tap Nxf1 Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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fitc rat anti mouse sca 1 antibody  (Miltenyi Biotec)
94
Miltenyi Biotec fitc rat anti mouse sca 1 antibody
List of antibodies for CC characterisation and differentiation.
Fitc Rat Anti Mouse Sca 1 Antibody, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti p115  (Proteintech)
93
Proteintech anti p115
List of antibodies for CC characterisation and differentiation.
Anti P115, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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flnb  (Proteintech)
93
Proteintech flnb
List of antibodies for CC characterisation and differentiation.
Flnb, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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abcb9  (Proteintech)
93
Proteintech abcb9
List of antibodies for CC characterisation and differentiation.
Abcb9, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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tap primary antibody  (Rockland Immunochemicals)
91
Rockland Immunochemicals tap primary antibody
List of antibodies for CC characterisation and differentiation.
Tap Primary Antibody, supplied by Rockland Immunochemicals, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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sca1 fitc  (Miltenyi Biotec)
94
Miltenyi Biotec sca1 fitc
( A ) Table represents the values obtained by analysing the local sequence alignment between the human and murine Lnc-Rewind transcripts. Data were produced by using the implementation of the Smith–Waterman algorithm available at http://www.ebi.ac.uk/Tools/psa/emboss_water/ . ( B ) Semiquantitative RT-PCR (sqRT-PCR) quantification of the human hs_Lnc-Rewind transcript in proliferating (GM) myoblasts from a healthy donor. Mature Gapdh was used as endogenous control. ( C ) FACS plot showing MuSC isolation strategy from WT mice. MuSCs are isolated, among the lineage (CD45/CD31/Ter119) negative cells, as <t>α7integrin+/Sca1−</t> cells (magenta box) (left and middle panels). The right plot shows the check purity of sorted MuSCs. ( D ) qRT-PCR quantification of Myog and Mck in C 2 C 12 and MuSC-derived myoblasts in growth (GM) and differentiated (DM) conditions. Data represent the mean ± SEM of three biological replicates and were normalized on Gapdh mRNA. ( E ) sqRT-PCR quantification of Lnc-Rewind, using different primers, in cytoplasmic (Cyt) and nuclear (Nuc) fractions from proliferating C 2 C 12 and MuSC-derived myoblasts. The quality of fractionation was tested with mature ( Gapdh ) and precursor ( pre-Gapdh ) RNAs. –rt represents the negative control ( F ) Representative 60X confocal images of MuSC-derived myoblasts cell cultures hybridized with probes set specific for Lnc-Rewind (upper panels) and for a human mRNA (dlc1), as negative control (neg ctrl) (bottom panels). Autofluorescence (gray) is shown with false colour to visualize the cell body. DAPI, 4’,6-diamidino-2-phenylindole (blue); scale bar: 25 μm. ( G ) Representative 60× confocal images of C 2 C 12 cell cultures hybridized with probes set specific for Lnc-Rewind (upper panels) and for a human mRNA (dlc1), as negative control (neg ctrl) (bottom panels). Autofluorescence (grey) is shown with false colour to visualize the cell body. DAPI, 4’,6-diamidino-2-phenylindole (blue); scale bar: 25 μm. Data information: *p<0.05, unpaired Student’s t-test. Figure 1—figure supplement 1—source data 1. Source data for .
Sca1 Fitc, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 94 stars, based on 1 article reviews
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anti sca 1 fitc antibody  (Miltenyi Biotec)
93
Miltenyi Biotec anti sca 1 fitc antibody
( A ) Table represents the values obtained by analysing the local sequence alignment between the human and murine Lnc-Rewind transcripts. Data were produced by using the implementation of the Smith–Waterman algorithm available at http://www.ebi.ac.uk/Tools/psa/emboss_water/ . ( B ) Semiquantitative RT-PCR (sqRT-PCR) quantification of the human hs_Lnc-Rewind transcript in proliferating (GM) myoblasts from a healthy donor. Mature Gapdh was used as endogenous control. ( C ) FACS plot showing MuSC isolation strategy from WT mice. MuSCs are isolated, among the lineage (CD45/CD31/Ter119) negative cells, as <t>α7integrin+/Sca1−</t> cells (magenta box) (left and middle panels). The right plot shows the check purity of sorted MuSCs. ( D ) qRT-PCR quantification of Myog and Mck in C 2 C 12 and MuSC-derived myoblasts in growth (GM) and differentiated (DM) conditions. Data represent the mean ± SEM of three biological replicates and were normalized on Gapdh mRNA. ( E ) sqRT-PCR quantification of Lnc-Rewind, using different primers, in cytoplasmic (Cyt) and nuclear (Nuc) fractions from proliferating C 2 C 12 and MuSC-derived myoblasts. The quality of fractionation was tested with mature ( Gapdh ) and precursor ( pre-Gapdh ) RNAs. –rt represents the negative control ( F ) Representative 60X confocal images of MuSC-derived myoblasts cell cultures hybridized with probes set specific for Lnc-Rewind (upper panels) and for a human mRNA (dlc1), as negative control (neg ctrl) (bottom panels). Autofluorescence (gray) is shown with false colour to visualize the cell body. DAPI, 4’,6-diamidino-2-phenylindole (blue); scale bar: 25 μm. ( G ) Representative 60× confocal images of C 2 C 12 cell cultures hybridized with probes set specific for Lnc-Rewind (upper panels) and for a human mRNA (dlc1), as negative control (neg ctrl) (bottom panels). Autofluorescence (grey) is shown with false colour to visualize the cell body. DAPI, 4’,6-diamidino-2-phenylindole (blue); scale bar: 25 μm. Data information: *p<0.05, unpaired Student’s t-test. Figure 1—figure supplement 1—source data 1. Source data for .
Anti Sca 1 Fitc Antibody, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 93 stars, based on 1 article reviews
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nxf3 specific antibody  (Proteintech)
93
Proteintech nxf3 specific antibody
( A ) Table represents the values obtained by analysing the local sequence alignment between the human and murine Lnc-Rewind transcripts. Data were produced by using the implementation of the Smith–Waterman algorithm available at http://www.ebi.ac.uk/Tools/psa/emboss_water/ . ( B ) Semiquantitative RT-PCR (sqRT-PCR) quantification of the human hs_Lnc-Rewind transcript in proliferating (GM) myoblasts from a healthy donor. Mature Gapdh was used as endogenous control. ( C ) FACS plot showing MuSC isolation strategy from WT mice. MuSCs are isolated, among the lineage (CD45/CD31/Ter119) negative cells, as <t>α7integrin+/Sca1−</t> cells (magenta box) (left and middle panels). The right plot shows the check purity of sorted MuSCs. ( D ) qRT-PCR quantification of Myog and Mck in C 2 C 12 and MuSC-derived myoblasts in growth (GM) and differentiated (DM) conditions. Data represent the mean ± SEM of three biological replicates and were normalized on Gapdh mRNA. ( E ) sqRT-PCR quantification of Lnc-Rewind, using different primers, in cytoplasmic (Cyt) and nuclear (Nuc) fractions from proliferating C 2 C 12 and MuSC-derived myoblasts. The quality of fractionation was tested with mature ( Gapdh ) and precursor ( pre-Gapdh ) RNAs. –rt represents the negative control ( F ) Representative 60X confocal images of MuSC-derived myoblasts cell cultures hybridized with probes set specific for Lnc-Rewind (upper panels) and for a human mRNA (dlc1), as negative control (neg ctrl) (bottom panels). Autofluorescence (gray) is shown with false colour to visualize the cell body. DAPI, 4’,6-diamidino-2-phenylindole (blue); scale bar: 25 μm. ( G ) Representative 60× confocal images of C 2 C 12 cell cultures hybridized with probes set specific for Lnc-Rewind (upper panels) and for a human mRNA (dlc1), as negative control (neg ctrl) (bottom panels). Autofluorescence (grey) is shown with false colour to visualize the cell body. DAPI, 4’,6-diamidino-2-phenylindole (blue); scale bar: 25 μm. Data information: *p<0.05, unpaired Student’s t-test. Figure 1—figure supplement 1—source data 1. Source data for .
Nxf3 Specific Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


List of antibodies for CC characterisation and differentiation.

Journal: International Journal of Molecular Sciences

Article Title: Human Wharton’s Jelly-Derived Mesenchymal Stem Cells Minimally Improve the Growth Kinetics and Cardiomyocyte Differentiation of Aged Murine Cardiac c-kit Cells in In Vitro without Rejuvenating Effect

doi: 10.3390/ijms20225519

Figure Lengend Snippet: List of antibodies for CC characterisation and differentiation.

Article Snippet: FITC Rat Anti-mouse Sca-1 Antibody (Clone D7) , 1:10 , FC , Miltenyi Biotec, Germany (130-102-297).

Techniques:

( A ) Table represents the values obtained by analysing the local sequence alignment between the human and murine Lnc-Rewind transcripts. Data were produced by using the implementation of the Smith–Waterman algorithm available at http://www.ebi.ac.uk/Tools/psa/emboss_water/ . ( B ) Semiquantitative RT-PCR (sqRT-PCR) quantification of the human hs_Lnc-Rewind transcript in proliferating (GM) myoblasts from a healthy donor. Mature Gapdh was used as endogenous control. ( C ) FACS plot showing MuSC isolation strategy from WT mice. MuSCs are isolated, among the lineage (CD45/CD31/Ter119) negative cells, as α7integrin+/Sca1− cells (magenta box) (left and middle panels). The right plot shows the check purity of sorted MuSCs. ( D ) qRT-PCR quantification of Myog and Mck in C 2 C 12 and MuSC-derived myoblasts in growth (GM) and differentiated (DM) conditions. Data represent the mean ± SEM of three biological replicates and were normalized on Gapdh mRNA. ( E ) sqRT-PCR quantification of Lnc-Rewind, using different primers, in cytoplasmic (Cyt) and nuclear (Nuc) fractions from proliferating C 2 C 12 and MuSC-derived myoblasts. The quality of fractionation was tested with mature ( Gapdh ) and precursor ( pre-Gapdh ) RNAs. –rt represents the negative control ( F ) Representative 60X confocal images of MuSC-derived myoblasts cell cultures hybridized with probes set specific for Lnc-Rewind (upper panels) and for a human mRNA (dlc1), as negative control (neg ctrl) (bottom panels). Autofluorescence (gray) is shown with false colour to visualize the cell body. DAPI, 4’,6-diamidino-2-phenylindole (blue); scale bar: 25 μm. ( G ) Representative 60× confocal images of C 2 C 12 cell cultures hybridized with probes set specific for Lnc-Rewind (upper panels) and for a human mRNA (dlc1), as negative control (neg ctrl) (bottom panels). Autofluorescence (grey) is shown with false colour to visualize the cell body. DAPI, 4’,6-diamidino-2-phenylindole (blue); scale bar: 25 μm. Data information: *p<0.05, unpaired Student’s t-test. Figure 1—figure supplement 1—source data 1. Source data for .

Journal: eLife

Article Title: Epigenetic regulation of Wnt7b expression by the cis -acting long noncoding RNA Lnc-Rewind in muscle stem cells

doi: 10.7554/eLife.54782

Figure Lengend Snippet: ( A ) Table represents the values obtained by analysing the local sequence alignment between the human and murine Lnc-Rewind transcripts. Data were produced by using the implementation of the Smith–Waterman algorithm available at http://www.ebi.ac.uk/Tools/psa/emboss_water/ . ( B ) Semiquantitative RT-PCR (sqRT-PCR) quantification of the human hs_Lnc-Rewind transcript in proliferating (GM) myoblasts from a healthy donor. Mature Gapdh was used as endogenous control. ( C ) FACS plot showing MuSC isolation strategy from WT mice. MuSCs are isolated, among the lineage (CD45/CD31/Ter119) negative cells, as α7integrin+/Sca1− cells (magenta box) (left and middle panels). The right plot shows the check purity of sorted MuSCs. ( D ) qRT-PCR quantification of Myog and Mck in C 2 C 12 and MuSC-derived myoblasts in growth (GM) and differentiated (DM) conditions. Data represent the mean ± SEM of three biological replicates and were normalized on Gapdh mRNA. ( E ) sqRT-PCR quantification of Lnc-Rewind, using different primers, in cytoplasmic (Cyt) and nuclear (Nuc) fractions from proliferating C 2 C 12 and MuSC-derived myoblasts. The quality of fractionation was tested with mature ( Gapdh ) and precursor ( pre-Gapdh ) RNAs. –rt represents the negative control ( F ) Representative 60X confocal images of MuSC-derived myoblasts cell cultures hybridized with probes set specific for Lnc-Rewind (upper panels) and for a human mRNA (dlc1), as negative control (neg ctrl) (bottom panels). Autofluorescence (gray) is shown with false colour to visualize the cell body. DAPI, 4’,6-diamidino-2-phenylindole (blue); scale bar: 25 μm. ( G ) Representative 60× confocal images of C 2 C 12 cell cultures hybridized with probes set specific for Lnc-Rewind (upper panels) and for a human mRNA (dlc1), as negative control (neg ctrl) (bottom panels). Autofluorescence (grey) is shown with false colour to visualize the cell body. DAPI, 4’,6-diamidino-2-phenylindole (blue); scale bar: 25 μm. Data information: *p<0.05, unpaired Student’s t-test. Figure 1—figure supplement 1—source data 1. Source data for .

Article Snippet: Cells were incubated with primary antibodies CD31-PE (MiltenyBiotec, 130111540; RRID: AB_2657296 ), CD45-PE (MiltenyBiotec, 139110797; RRID: AB_2658218 ), Ter119-PE (MiltenyBiotec, 130112909; RRID: AB_2654115 ) 1:25; Sca1-FITC (MiltenyBiotec, 130116490; RRID: AB_2751322 ) 1:50; α7Integrin-APCVio770 (MiltenyBiotec, 130095212; Custom) 1:20 for 45 min on ice diluted in HBSS containing 0.2% BSA, 1% penicillin-streptomycin, and 1% DNAse I.

Techniques: Sequencing, Produced, Reverse Transcription Polymerase Chain Reaction, Control, Isolation, Quantitative RT-PCR, Derivative Assay, Fractionation, Negative Control

Journal: eLife

Article Title: Epigenetic regulation of Wnt7b expression by the cis -acting long noncoding RNA Lnc-Rewind in muscle stem cells

doi: 10.7554/eLife.54782

Figure Lengend Snippet:

Article Snippet: Cells were incubated with primary antibodies CD31-PE (MiltenyBiotec, 130111540; RRID: AB_2657296 ), CD45-PE (MiltenyBiotec, 139110797; RRID: AB_2658218 ), Ter119-PE (MiltenyBiotec, 130112909; RRID: AB_2654115 ) 1:25; Sca1-FITC (MiltenyBiotec, 130116490; RRID: AB_2751322 ) 1:50; α7Integrin-APCVio770 (MiltenyBiotec, 130095212; Custom) 1:20 for 45 min on ice diluted in HBSS containing 0.2% BSA, 1% penicillin-streptomycin, and 1% DNAse I.

Techniques: Sequencing, Control, Clone Assay, SYBR Green Assay, Magnetic Beads, Recombinant, Software